Automated carboxy-terminal sequence analysis of peptides
نویسندگان
چکیده
منابع مشابه
Functional analysis of carboxy-terminal deletion mutants of c-Myb.
The c-myb gene is implicated in the differentiation and proliferation of hematopoietic cells. Truncations of the N and/or C terminus of c-Myb, found in v-Myb, can potentiate its transforming ability. Two negative regulatory subregions, located in the C terminus, were mapped previously by using GAL4-c-Myb fusion proteins in transient transfection assays for the transcriptional activation of a GA...
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Peptide and protein molecules are composed of amino acid molecules covalently coupled end-to-end through peptide bonds, resulting in linear sequences of the amino acid residues. These polypeptide molecules have two chemically distinguishable termini or ends, identified as the amino (N) terminus and the carboxy (C) terminus. The N-terminal and C-terminal amino acid residues define the ends of th...
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Activation of glutamate decarboxylase (GAD) by calcium-bound calmodulin (CaM) is required for normal plant growth through regulation of gamma-aminobutyrate and glutamate metabolism. The interaction of CaM with the C-terminal domain of GAD is believed to induce dimerization of the enzyme, an event implicated for Ca(2+)-dependent enzyme activation. Here, we present the solution structure of CaM i...
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Fibrillins are large cysteine-rich glycoproteins (~350 kDa) which form the molecular scaffold of a class of beaded microfibrils that are key structural elements of dynamic connective tissues (Kielty and Shuttleworth, 1995). These microfibrils are extensible polymers which act as a structural lattice for elastin deposition during elastic fibre formation (Mecham and Heusar, 1991). Linkage of the ...
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In this protocol, we describe an approach in which two-dimensional electrophoresis (2DE)-separated proteins are guanidinated in-gel prior to enzymatic cleavage. In contrast to previously described techniques, this procedure allows the extracted tryptic peptides to be N-terminally sulfonated without any further sample purification. The protocol was applied on a proteomic study of 2DE-separated p...
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ژورنال
عنوان ژورنال: Protein Science
سال: 1992
ISSN: 0961-8368
DOI: 10.1002/pro.5560010108